Fluorescent Polymers – Markers for Biomedical Applications
G. CARAENE, M. ALBULESCU, R. ALBULESCU, M. MOSCOVICI, T. BURIANA,
A. ALBULESCU, L. BUD
Abstract. The development of luminescent polymers as markers for the
study of biochemical and biological processes or as biomaterials for
medicinal use is one of the main directions in organic luminofore
research. For the present study we used pullulan, a polymer with special
physical properties, functionalized with a dansyl-like luminofore which
was synthetized for this purpose. The polymer was modified to contain
organic functions capable of reacting with the dansyl derivative –
N-methyl-N-(5-dimethylaminonaphtalene-1-sulphonyl)-ethanolamine
(N-methyl-N-dansylethanolamine), obtained from the reaction of dansyl
chloride and N-methylethanolamine. The fluorescence spectrum was
determined in methanolic sollution, in order to establish the fluorescent
properties of the synthetized derivative. Also, a pharmacological study
was performed, in order to evaluate the cytotoxicity of the functionalized
polymer. The results suggest that the substance is practically non-cytotoxic
and therefore usable for a wide area of biological applications. |