S-layer of Haloferax sp. GR 2 (JCM 13922): Isolation, Characterization and
Binding to Silicon Nanostructurated Substrates
L. DUMITRU, G. TEODOSIU POPESCU, M. ENACHE, R. COJOC, I. KLEPS, T.
Abstract. Crystalline cell surface layers (S-layers) composed of
planar assemblies of protein or glycoprotein subunits are one of the most
commonly observed cell envelope structures of Bacteria and Archaea.
Isolated S-layer subunits of microorganisms are able to assemble into
monomolecular arrays either in suspension, at liquid-surface interfaces,
including lipid films, on liposomes and on solid supports. These
characteristic features of S-layers have led to various applications in
biotechnology, vaccine development, diagnosis, biomimetics and molecular
nanotechnology. Our investigations were done by an extremely halophilic
archaeaon isolated from a hypersaline lake in Prahova County, Romania. The
studies were focused on: isolation of S-layer, indirect reveal by
biochemical characterization and examination in transmission electron
microscope as direct method for visualize of protein lattice. Preliminary
investigations related to the binding of S-layer to some silicon
nanostructurated substrates were performed, also.