INVESTIGATION OF THE BINDING CONSTANT OF BIOCOMPATIBLE GOLD NANOPARTICLES TO BOVINE SERUM ALBUMINE USING FLUORESCENCE AND LSPR SPECTROSCOPY
M. IOSIN, F. TODERAS, P. L. BALDECK, S. ASTILEAN

Abstract. We investigate the adhesion of Bovine Serum Albumine (BSA) to gold nanoparticles by measuring the spectral shift of Localized Surface Plasmon Resonance (LSPR) and the fluorescence quenching of tryptophan residue in BSA after protein binding to the surface of metal. The spectroscopic data allow the calculation of constant binding (Kb) between the protein and colloidal gold and the numbers of specific binding sites.