Chaperones Improve Kinetics of the Denatured Enzymes
A. C. Laslo, E. Ganea
Abstract. The kinetics of artificial chaperone-assisted refolding process
of the two chemically–denatured enzymes, leucine aminopeptidase and
xanthine oxidase was investigated by evaluation of two kinetics
parameters: Michaelis–Menten constant and maximum velocity. The kinetics
of the recovered enzymes was significantly improved by chaperones, with
some specific features of each enzyme.